Yini okufanele uyinake lapho usebenzisa imitha ye-BOD5?

Yini okufanele uyinake lapho usebenzisa i-I-BOD analyzer:
1. Ukulungiselela ngaphambi kokuhlolwa
1. Vula amandla e-incubator ye-biochemical amahora angu-8 ngaphambi kokuhlolwa, futhi ulawule izinga lokushisa ukuze lisebenze ngokujwayelekile ku-20°C.
2. Faka amanzi okuhlola e-dilution, amanzi okujova kanye namanzi e-inoculation dilution ku-incubator futhi uwagcine emazingeni okushisa angashintshi ukuze asetshenziswe kamuva.
2. Ukwelashwa kwesampula yamanzi
1. Lapho inani le-pH lesampula lamanzi lingekho phakathi kuka-6.5 no-7.5; okokuqala wenze ukuhlolwa okuhlukile ukuze kutholwe ivolumu edingekayo ye-hydrochloric acid (5.10) noma isixazululo se-sodium hydroxide (5.9), bese unciphisa isampula, kungakhathaliseki ukuthi kukhona imvula. Uma i-acidity noma i-alkalinity yesampula yamanzi iphezulu kakhulu, i-alkali yokugxilwa okuphezulu noma i-asidi ingasetshenziswa ukuze kuncishiswe amandla, kuqinisekiswe ukuthi inani alikho ngaphansi kuka-0.5% wevolumu yesampula yamanzi.
2. Ngamasampula amanzi aqukethe inani elincane le-chlorine yamahhala, i-chlorine yamahhala izonyamalala ngokuvamile ngemva kokushiywa amahora angu-1-2. Kumasampula amanzi lapho i-chlorine yamahhala ingenakunyamalala phakathi nesikhathi esifushane, inani elifanele lesisombululo se-sodium sulfite lingengezwa ukuze kukhishwe i-chlorine yamahhala.
3. Amasampula amanzi aqoqwe emanzini anamazinga okushisa aphansi amanzi noma amachibi ane-eutrophic kufanele ashiselwe ngokushesha afike cishe ku-20°C ukuze kukhishwe umoya-mpilo oncibilikisiwe one-supersaturated emasampuli amanzi. Uma kungenjalo, imiphumela yokuhlaziya izoba phansi.
Uma uthatha amasampula emanzini anamazinga okushisa aphezulu amanzi noma izindawo zokukhipha amanzi angcolile, kufanele apholise ngokushesha aze afike ku-20 ° C, ngaphandle kwalokho imiphumela yokuhlaziya izoba phezulu.
4. Uma isampula lamanzi elizohlolwa lingenawo ama-microorganisms noma umsebenzi we-microbial onganele, isampula kufanele igonywe. Njengezinhlobo ezilandelayo zamanzi angcolile ezimbonini:
a. Amanzi angcolile ezimbonini angazange aphathwe nge-biochemical;
b. Ukushisa okuphezulu nokucindezela okukhulu noma amanzi angcolile ahlanzekile, ukunakwa okukhethekile kufanele kukhokhwe emanzini angcolile avela embonini yokucubungula ukudla kanye nendle yasekhaya evela ezibhedlela;
c. Amanzi angcolile ezimboni ane-asidi ne-alkali;
d. Amanzi angcolile ezimbonini anenani eliphakeme le-BOD5;
e. Amanzi angcolile ezimbonini aqukethe izinto ezinobuthi njengethusi, i-zinc, umthofu, i-arsenic, i-cadmium, i-chromium, i-cyanide, njll.
Amanzi angcolile asezimbonini angenhla adinga ukuphathwa ngama-microorganisms anele. Imithombo yama-microorganisms imi kanje:
(1) Isithako esinamandla sendle ehlanzekile yasendlini engagezwanga esibekwe ku-20°C amahora angama-24 kuya kwangama-36;
(2) Uketshezi olutholakala ngokuhlunga isampula ngephepha lokuhlunga ngemva kokuphothulwa kokuhlolwa kwangaphambilini. Lolu ketshezi lungagcinwa ku-20℃ inyanga eyodwa;
(3) Ukungcola okuvela ezikhungweni zokukhuculula indle;
(4) Amanzi omfula noma ichibi aqukethe indle yasemadolobheni;
(5) Izinhlobo zebhaktheriya ezinikezwe nethuluzi. Kala u-0.2g we-bacterial strain, uthele ku-100ml wamanzi ahlanzekile, ugovuze ngokuqhubekayo kuze kube yilapho izigaxa zihlakazeka, zifake ku-incubator ngo-20 ° C futhi zivumele zime amahora angu-24-48, bese uthatha i-supernatant.